The effect of urokinase on the expression of MMP-9 in human endothelial cell and its mechanism
- VernacularTitle:尿激酶对人血管内皮细胞MMP-9表达的影响及机制
- Author:
Shutang ZHANG
- Publication Type:Journal Article
- Keywords:
Endothelialcell;
Urokinase;
Matrixmetalloproteinase9
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of urokinase on the expression of Matrix metalloproteinase 9 (MMP-9) in human endothelial cell and its mechanism. Methods: The human umbilical vein endothelial cell cultured in normal condition were divided into five groups: the first group was blank control,the second group was given urokinase,the third,fourth and fifth groups were treated with MEK inhibitor PD98059,NF-?B inhibitor PDTC,and AP-1 inhibitor Curcumin,respectively before urokinase was added in. The expression of MMP- 9 mRNA and protein in all the groups were detected by RT-PCR and Laser-Confocus respectively. Results: The mRNA and protein expression of MMP-9 in the second group were increased obviously,as compared with the first group. Compared with the second group,the mRNA and protein expression of MMP-9 in the third,fourth,and fifth groups were decreased significantly. Conclusion: Urokinase can upregulate the MMP-9 mRNA and protein expression of human endothelial cell. The mechanism is related to the Ras-Raf-MEK1/2-ERK1/2 signal transduction pathway,also to NF-?B and AP-1.
