Preparation of doxorubicin liposome and their effect on human MCF-7
- VernacularTitle:阿霉素脂质体的制备及对MCF-7/DOX作用的考察
- Author:
Ke YAN
- Publication Type:Journal Article
- Keywords:
DOXliposome;
(NH4)2SO4gradientmethod;
Multidrugresistance;
MCF-7
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare doxorubicin liposome and investigate its effect on human MCF-7 in vitro. Methods:DOX liposome was prepared by(NH4)2SO4-gradient method.The MTT method was used to test cytotoxicity and reverse multidrug resistance of DOX liposome on MCF-7/DOX. Results:DOX was encapsulated by 90.77%. The results of liposome MTT experiment suggested that IC50 of free DOX was 4 times bigger than DOX liposome.The DOX liposomes’reversing effect on MDR was 4 times as much as that of free DOX. Conclusion:The method of(NH4)2SO4 gradient is suitable for producing DOX liposome and it has better cytotoxicity and reversing MDR effect on MCF-7 than free DOX.
