Methodological Study of Puerarin Content in Radix Puerariae by Coulomb Electrochemical Determination
- VernacularTitle:库仑电化学法测定葛根中葛根素的方法学研究
- Author:
Zhenhua WANG
;
Lin SUN
;
Zhiqiang WEI
;
Jun TIAN
;
Qiuling KONG
;
Yan PAN
- Publication Type:Journal Article
- Keywords:
Puerarin;
Content determination;
Electrochemical determination;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the electrochemical determination method for puerarin in Radix Puerariae.Methods The electrochemical behavior and content determination of puerarin were investigated by using coulomb array detector.Results In phosphate buffer with PH 4.32,puerarin showed good linearity in the range of 0.16 ng～ 162 ng.The regression equation was A=1444.7C+ 88.635,r=0.9998(n=7),the average recovery was 96.88 %,and RSD was 0.08 %.Conclusion This method is simple and rapid,and the result is accurate and reliable.It can be used for the determination of puerarin content and biological samples.
