Determination of Astragaloside Ⅳ in Fufang Buqi Yangxue Oral Solution by RP-HPLC
- VernacularTitle:RP-HPLC法测定复方补气养血口服液中黄芪甲苷的含量
- Author:
Haiyun ZHAI
;
Yanhong WU
;
Qinghua HUANG
;
Yuanxin LI
;
Xujiang YUAN
- Publication Type:Journal Article
- Keywords:
Fufang Buqi Yangxue Oral Solution;
Astragaloside Ⅳ;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for determination of astragaloside Ⅳ in Fufang Buqi Yangxue Oral Solution by RP-HPLC.Methods The content of astragaloside Ⅳ was determined by a HPLC system under the following conditions:the chromatographic column was Agilent C18(150 mm? 4.60 mm,5? m),the mobile phase was acetonitrile-water(60:40),the flow rate was 1.0 mL? min-1,and the detection wavelength was at 203 nm.Results Astragaloside Ⅳ showed good linearity in the range of 0.34～ 3.40 ?g(r=0.999 9).The average recovery was 100.8 %,and RSD was 1.47 %(n=6).Conclusion The HPLC method is simple and reproducible,and can be used for quality control of Fufang Buqi Yangxue Oral Solution.
