Determination of tanshinone Ⅱ_A and salvianolic acid B in Chaidan Jieyu Granules by HPLC gradient elution
- VernacularTitle:HPLC梯度洗脱法同时测定柴丹解郁颗粒中丹参酮Ⅱ_A、丹酚酸B含量
- Author:
Mei HU
;
Qun GAO
;
Jing TAN
- Publication Type:Journal Article
- Keywords:
Chaidan Jieyu Granule;
tanshinone Ⅱ_A;
salvianolic acid B;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine tanshinone Ⅱ_A and salvianolic acid B by HPLC gradient elution. METHODS: HPLC was used with Hypersil-ODS C_(18) column(150 mm?4.6 mm,5 ?m).The mobile phase consisted of methanol-water 0~20 min(35-90:6510),20-25 min(90-95:10-5),25-30 min(95:5)gradient elution.The flow rate was 1.0 mL/min with column temperature at 30 ℃.The UV detection wavelength was at 286 nm in 0-20 min and 270 nm in 20-30 min,respectively. RESULTS: The linear range of tanshinone Ⅱ_A and salvianolic acid B were in the range of 0.014 448-0.096 32(r=0.999 97), and 0.198 6-3.972 ?g(r=0.999 94),respectively.The average recoveries were 99.62%(RSD=2.28%) and 100.58%(RSD=2.5%),respectively.(CONCLUSION:) The method is accurate、sensitive、reproducible,and suitable for the quality control of Chaidan Jieyu Granules.
