The Effect of Glutathione on High Dose Cisplatin-Induced Cellular Toxicity in Non-small Cell Lung Cancer Cell Lines.
10.4046/trd.2002.52.5.463
- Author:
Seoung Il LEE
1
;
Gwi Beom BOO
;
Dai Yong JANG
;
Ki Young CHUNG
;
Jeoung Gyun SEO
;
Byeong Lai LEE
;
Jong Hoon CHUNG
Author Information
1. Department of Internal Medicine, College of Medicine, Chosun University, Kwangju, Korea. silee@mail.chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Glutathione;
High-dose cisplatin;
Non-small cell lung cancer cell lines;
Cellular toxicity
- MeSH:
Adenocarcinoma;
Carcinoma, Non-Small-Cell Lung*;
Carcinoma, Squamous Cell;
Cell Line*;
Cells, Cultured;
Cisplatin;
Epithelial Cells;
Glutathione*;
Lung;
Sulfur Compounds
- From:Tuberculosis and Respiratory Diseases
2002;52(5):463-474
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: This study was designed to examine how glutathione, one of the nucleophilic sulfur compounds, effects the cisplatin cellular toxicity in the non-small cell lung cancer cell lines and normal lung epithel ial cell line. METHODS: Three cultured cell lines, the lung adenocarcinoma cell(NCL-H23), the lung squamous carcinoma cell (SK-MES-1) and the normal lung epithelial cell(L-132) line were exposed to various concentrations of cisplation with or without glutathione. The relative viability was estimated as a means of measuring the cisplatin cellular toxicity using the MTT method. RESULTS: In NCL-23, the response to cisplatin was sensitive but glutathione markedly increased the relative survival of the tumor cells by removing the antitumor effect of cisplatin. In both SK-MES-1 and L-132, the responses to cisplatin were less sensitive, and the chemoprotective effect of glutathione compared to an equal cisplatin dose was signigicantly higher in L-132 than in SK-MES-1(p<0.05). CONCLUSION: The protective effects of glutathione on cisplatin-induced cellular toxicity is more signigicant in normal lung epithelial cells than in squamous carcinoma cells.
