Effect of Changji'an Prescription on T Lymphocytes and 5-Hydroxytryptamine in Rats with Diarrhea Predominant Irritable Bowel Syndrome
- VernacularTitle:肠激安方对腹泻型肠易激综合征模型大鼠T淋巴细胞和5-羟色胺的影响
- Author:
Hongmei TANG
;
Yinghua HUANG
;
Detang LI
;
Zhenwen QIU
;
Xue YAN
- Publication Type:Journal Article
- Keywords:
CHANGJI'AN PRESCRIPTION/pharmacology;
IRRITABLE BOWEL SYNDROME/TCD therapy;
IRRITABLE BOWEL SYNDROME /immunology;
COLON/pathology;
DISEASE MODELS,ANIMAL;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Changji'an Prescription(CP) on T lymphocytes count and 5-hydroxytryptamine(5-HT) secretion in rats with diarrhea predominant irritable bowel syndrome(IBS).Methods Forty-six neonatal SD rats were randomized into normal group,model group,low-,middle-and high-dose CP groups(0.94,1.88 and 3.76g?kg-1?d-1 respectively),and Bupi Yifei Pills group(6.76g?kg-1?d-1).Continuous internal rectal stimulation with acetate was used to induce the rat model of intestinal hypersensitive IBS.Flow cytometer was used for the quantitative analysis of peripheral levels of T lymphocytes subsets CD+3,CD+4 and CD+8,and immunohistochemical method was used to observe 5-HT-positive cells number and the immunostaining intensity in the bowels of rats.Results In the model group,the defecation volume and the intestinal sensitivity were increased,CD+4 level and CD+4/CD+8 decreased,CD+8 level increased,5-HT-positive cells number and the immunostaining intensity elevated(P
