Effect of Serums Containing Xuefuzhuyu Capsules and Xuesetong Capsules on the Proliferation of Vascular Smooth Muscle Cells Induced by Lysophosphatidie Acid
- VernacularTitle:血府逐瘀胶囊、血塞通胶囊对溶血磷脂酸诱导的血管平滑肌细胞增殖的影响
- Author:
Jie WANG
;
Yingkun FU
;
Suping FANG
- Publication Type:Journal Article
- Keywords:
Xuefuzhuyu capsule;
Xuesetong capsule;
lysophosphatidie acid;
vascular smooth muscle cell;
drug-containing serum
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the serums containing Xuefuzhuyu capsules and Xuesetong capsules on the proliferation of vascular smooth muscle cells (VSMC) induced by 1ysophosphatidie acid (LPA). Methods Rat VSMCs were obtainded by tissue-piece inoculation and divided into seven groups:blank serum group, Xuefuzhuyu (Xuesetong) capsules-containing serum group, LPA+ Xuefuzhuyu (Xuesetong, captopril) containing serum group and LPA+blank serum group. Effects of different groups on the proliferation of VSMCs was evaluated by MTT colorimetry. Analysis of cell cycle and DNA content were performed by flow cytometry. Results The optical density (OD) and the S phase fraction (SPF) of LPA+blank serum group and Xuefuzhuyu capsules-containing serum group were more increased than the blank serum group (P 0.05, P 0.05). Conclusion Xuefuzhuyu capsules and LPA promoted the proliferation of VSMCs in vitro. Xuefuzhuyu and Xuesetong capsules can inhibit the proliferation induced by LPA, and effect of Xuesetong capsules was greater. Part of the mechanism may be associated with the inhibition of DNA synthesis and preventing the transition from G0/G1 to S phase of VSMCs.
