Determination of Caffeic Acid and Rosmarinic Acid in Prunella vulgaris from Different Habitat by HPLC
- VernacularTitle:高效液相色谱法测定不同产地夏枯草中咖啡酸和迷迭香酸的含量
- Author:
Wen QIN
;
Lanzhen ZHANG
;
Yinying BA
- Publication Type:Journal Article
- Keywords:
Prunella vulgaris;
caffeic acid;
rosmarinic acid;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination and comparison of the content of caffeic acid and rosmarinic acid in Prunella vulgaris from different habitat. Methods The determination was carried out with Waters Zorbax SunFire C18 (4.6 mm?150 mm, 5 ?m), gradient MeOH-0.1% acetic acid solution was taken as the mobile phase at a flow rate of 1.0 mL/min, and the detection wavelength was at 330 nm. Results The linear range of caffeic acid was 0.099 2～0.496 0 ?g (r=0.999 9). The average recovery rate of caffeic acid was 99.75% (RSD=2.27%, n=5). The content of caffeic acid in Prunella vulgaris from different habitat ranged from 0.02% to 0.05%. The linear range of rosmarinic acid was 0.828 8～4.144 0 ?g (r=0.999 8), The average recovery rate of rosmarinic acid was 101.37% (RSD=1.19%, n=5). The content of rosmarinic acid in Prunella vulgaris from different habitat ranged from 0.09% to 0.20%. Conclusion The content of caffeic acid and rosmarinic acid in Prunella vulgaris from different habitat varied greatly.
