Determination of Three Kinds of Phenylpropanoids in Stem Rind of Daphne giraldii by HPLC
- VernacularTitle:高效液相色谱法测定祖师麻中3种苯丙素成分的含量
- Author:
Yunyun JING
;
Jinjun SHAN
;
Jia LIU
- Publication Type:Journal Article
- Keywords:
Zushima;
HPLC;
daphnetin;
7-OH coumarin;
syringin;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of daphnetin, 7-OH coumarin and syringing in Zushima. Methods The separation was performed on YMC-Pack ODS (4.6 mm?250 mm, 5 ?m) column with a mobile phase consisting of methanol-0.5% acetic acid-water (19︰8︰1) as gradient elution at the flow rate of 1.0 mL/min. The dual detective wavelength was set at 327 nm and 266 nm. The column temperature was 30 ℃. Results Baseline separation was achieved for the analysis of the three index components. The average recoveries were 98.4%～100.2%. Conclusion This method is convenient, rapid and accurate. It can be used for quality control in production of Zushima.
