Determination of Theobromine in Compound Theophylline and Ephedrine Tablet by HPLC
- VernacularTitle:高效液相色谱法测定复方茶碱麻黄碱片中可可碱的含量
- Author:
Haixia LIU
;
Qingmiao WU
- Publication Type:Journal Article
- Keywords:
Compound Theophylline and Ephedrine Tablet;
HPLC;
theobromine;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of theobromine in Compound Theophylline and Ephedrine Tablet. Method HPLC method was adopted on Kromasil C18 column (250 mm? 4.6 mm, 5 ?m), and temperature was set at 45 ℃. The mobile phase consisted of 0.05 mol/L KH2PO4- Methanol-Triethanolamine (77∶23∶0.2), with flow rate of 0.8 mL/min. The UV detection wavelength was set at 215 nm. Results The calibration curves of theobromine was linear in the range of 0.3 094～0.722 ?g (r=0.999 96), ant the average recovery was 100.49% (n=9) with RSD=1.1%. Conclusion This method is simple, rapid, sensitive and accurate, and can be used for determination of theobromine in Compound Theophylline and Ephedrine Tablet.
