Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation
- VernacularTitle:改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素
- Author:
Xiaoling LIU
- Publication Type:Journal Article
- Keywords:
Toosendanin;
Transonic extraction;
High performance liquid chromatograph(yHPLC)
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an improved transonic extraction-high performance liquid chromatography(HPLC)method for separating and detecting toosendanin(TSN)in Melia toosendanin extractive.Methods:Transonic alcohol-chloroform extraction method was used to extract TSN from the bark of Melia toosendanin sieb.et zuccc.The content determination of TSN was performed on an Agilent XDB C18 Column(4.6?250 mm,5?m).Elution was performed using 45%acetonitrile-water solution as the mobile phase at a1flow rate of 1ml/min.Detection was performed at 215 nm wavelength.Results:The linear regression equation for peak area-TSN concentration(4.64～88.2?g/ml)was:A=7.616 2C-2.567 7,r=0.999 6;mean recovery of standard substance was 100.83%;RSD of precision test was 1.42%;RSD of stability test was 2.26%.Conclusion:The method is simple,rapid,stable and roducible,which can be used as a method for preparation and identification TSN of Melia toosendanin extractive in laboratory.
