Establishment of the rat model of chronic allograft nephropathy
- VernacularTitle:大鼠慢性移植肾肾病模型的建立
- Author:
Yaxiong TANG
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
Rat;
Chronic rejection;
Model
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the pathology,therapy and prevention of the chronic rejection after rat renal transplantation by establishing a simple and feasible allograft nephropathy model.Methods:We used Fisher rats as donors and Lewis rats as recipients.After the left kidney of the donors was removed,the inferiorvena of the donor was anastomosed end-to-end with the left renal vein of the recipient,and the donor's abdominal aorta was anastomosed end-to-side with that of the recipient with the unaided eyes to carry out renal allograft in prime position.The recipients were given ciclosporin oral solution10 mg/(kg.d)by gavage for 10 days after transplantation,and the pathological changes of the recipient's transplant kidney would be respectively observed after 4 weeks.Results:The renal allograft in prime position were finished within 2.5 hours,and the achievement ratio was 97.5%.The pathological changes of the transplanted kidney began to appear 4 weeks after transplantation then the chronic rejection could be seen after 12 weeks.Conclusion:This method was simple and direct-viewing,and reduced microsurgery equipment,which successfully established rat renal transplantation model of chronic allograft nephropathy.
