Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation
- VernacularTitle:海马脑片氧糖剥离模型中血晶素对脑红蛋白表达的影响
- Author:
Li WANG
- Publication Type:Journal Article
- Keywords:
Hippocampal organotypic tissue culture(sHOTC);
Oxygenl/glucose deprivation(OGD);
Neuroglobin;
Hemin
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the neuroprotective role of hemin and its effect on the expression of neuroglobin in the hippocampal organotypic tissue cultures(HOTC)after combined oxygen/glucose deprivation(OGD).Methods:SD rats,8~10-days old,were sacri-ficed for brain.HOTC were made and randomly divided into three groups:HOTC,HOTC undergoing OGD(OGD),HOTC under- going OGD following pretreatment with hemin(Hemin+OGD). The expression of neuroglobin was detected by immunohisto-chemical technique and computer-assisted image analysis.Results:In OGD group,hippocampal organotypic tissue lost normal structure and neurons were damaged severely.Pretreatment with hemin could effectively reverse the damage of the neurons.The expression of neu- roglobin in the Hemin+OGD group were significantly stronger than that in the OGD group.Conclusion:Hemin has protective effect on injury induced by OGD in rat hippaocampal slices,which is associated with enhanced expression of neuroglobin.
