Method for rapid analysis of puerarin in Xintong Oral Liquid by AOTF-near infrared spectroscopy
- VernacularTitle:一种快速测定心通口服液中葛根素含量的新方法
- Author:
Ning WANG
;
Hong WEI
;
Shaosong CAI
;
Zhengqi LI
- Publication Type:Journal Article
- Keywords:
acousto-optic tunable filter;
near-infrared spectroscopy;
Xintong Oral Liquid;
PLS1 algorithm;
puerarin;
rapid analysis
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a new method of rapid analysis of puerarin in Xintong Oral Liquid by acousto-optic tunable filter-nearinfrared spectroscopy. METHODS: HPLC was used as a reference method to determine puerarin content in Xintong Oral Liquid. Calibration model based on partial least squares(PLS1) algorithm was developed to correlate the spectra and the corresponding values determined by the reference methods. RESULTS: The root mean square error of prediction(RMSEP) of the model for puerarin was 0.137 1. The determination coefficient was R～2=0.984 5. The correlation coefficient of the true value and predication value from validation was r～2=0.996 4. CONCLUSION: The method is a quick, simple and low cost assay and able to be used in quantitative analysis of Xintong Oral Liquid.
