Determination of sinomenine and chelidonine in Tong’an Injection by HPLC
- VernacularTitle:HPLC同时测定痛安注射液中青藤碱和白屈菜碱含量
- Author:
Lanlan XU
;
Wei XIAO
;
Junhua HU
;
Qiang SHANG
;
Jianping QIN
- Publication Type:Journal Article
- Keywords:
Tong’an Injection;
HPLC;
sinomenine;
chelidonine
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the contents of sinomenine and chelidonine in Tong’an Injection(Caulis sinomenii, Chelidonium majus Linn, etc). METHODS: HPLC was used. The conditions included the gradient elution with methanol-0.1% triethylamine. The detection wavelength was set at 280 nm. RESULTS: The calibration curve was linear in the range of 162-1 620 ?g for the sinomenine and the range of 35-350 ?g for chelidonine, respectively. The average recovery for sinomenine was 99.56% and the relative standard deviation was 0.41%(n=5). The average recovery for chelidonine was 99.46% and the relative standard deviation was 0.62% (n=5). CONCLUSION: The method is simple, rapid and specialty. It can be used for the determination of sinomenine and chelidonine in Tong’an Injection.
