Effect of Tongbiling on VEGF mRNA Expression in Rheumatoid Arthritis Synovial Cell
- VernacularTitle:通痹灵对类风湿关节炎滑膜细胞血管内皮生长因子mRNA的影响
- Author:
Qingchun HUANG
;
Yongliang CHU
;
Ligang JIE
- Publication Type:Journal Article
- Keywords:
Tongbiling;
rheumatoid arthritis;
synovial cell;
vascular endothelial growth factor
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effect of Tongbiling (TBL) on VEGF mRNA expression in RA synovlcytes cultured in vitro and explore its mechanism. Method Synovial cells from the knee joint in patient with RA were digested, divided and separately cultured after arthroscopy. Blank controls:DMEM culture containing 10% FBS in saline;Low-dose admission group of TBL:DMEM culture containing 10% FBS in 50 mg/L TBL;High-dose admission of TBL:DMEM culture containing 10% FBS in 200 mg/L TBL. RNA were distilled. Expression of VEGF mRNA were detected with RT-PCR. Result Compared with control group, expression of VEGF mRNA in low and high-dose admission groups of TBL both decreased (P
