Effects of the Mixture of Ferulic Acid and Paeoniflorin on PC12 Cells Injury
- VernacularTitle:阿魏酸和芍药苷混合剂对PC12细胞损伤的影响
- Author:
Lijun MA
;
Zhenhua MIAO
;
Wei MA
- Publication Type:Journal Article
- Keywords:
ferulic acid;
paeoniflorin;
PC12 cells
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effects of the mixture of ferulic acid (AF) and paeoniflorin on PC12 cells injury by FeSO4/H2O2 in vitro, and explorer its mechanism. Methods The cell injury model was established with FeSO4/H2O2 for 24 h. The process of the growth and morphology of the cells were observed under phase contrast microscope in vitro. The survival rate of the cells was determined by MTT method. The content of MDA and SOD activity was measured respectively by thiobarbituric acid and xanthine oxidase method. Results Compared with FeSO4/H2O2, the mixture of ferulic acid (AF) and paeoniflorin enhanced the PC12 cells survival rate and viability, reduced MDA content and increased SOD activity. Conclusion The mixture of 5～50 ?mol/L AF and 1～10 ?mol/L paeoniflorin exerted protective effects on PC12 cells injury induced by FeSO4/H2O2, which maybe related to free radical scavenging activity and lipid peroxidation process being inhibited.
