Determination of Osthol in Compound Shechuang Tincture by HPLC
- VernacularTitle:高效液相色谱法测定复方蛇床酊中蛇床子素的含量
- Author:
Jilian SHI
;
Qun HE
;
Ziy YANG
- Publication Type:Journal Article
- Keywords:
Compound Shechuang Tincture;
osthol;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the determination method for osthol in Compound Shechuang Tincture. Methods Hypersil ODS column was used and methanol-water (70∶30) was used as a mobile phase. The flow rate was at 1.0 mL/min and the detection wave-length was at 322 nm. Results Osthol had a good linear relationship between 0.018～0.126 ?g (r =0.999 9). The average recovery was 98.55% and RSD was 1.45%. Conclusion This method is simple, rapid and accurate. It can be applied to determining osthol in Compound Shechuang Tincture.
