Determination of Six Phendic Acids in Mailuoning Injection by RP-HPLC
- VernacularTitle:反相高效液相色谱法同时测定脉络宁注射液中6种酚酸类成分的含量
- Author:
Hui ZHANG
;
Xiang LI
;
Hongyan ZHOU
- Publication Type:Journal Article
- Keywords:
Mailuoning injection;
neochlorogenic acid;
chlorogenic acid;
cryptochlorogenic acid;
caffeic acid;
3,5-Dicaffeoylquinic acid;
3,4- Dicaffeoylquinic acid;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination the contents of six phendic acids in Mailuoning injection by HPLC at same time. Methods Separation was performed on a Kromasil-C18 (4.6 mm? 250 mm, 5 ?m) column with mobile phase consisting of 0.1% phosphoric acid-water and Acetonitrile with gradient elute at the flow rate of 1 mL/min. The UV detection wavelength was set at 327 nm and the column temperature was set at 25 ℃. The sample size was 20 ?L. Results The standard cures of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 3,5-Dicaffeoylquinic acid, 3,4- Dicaffeoylquinic acid were linear within the contentration range of 0.010 90～0.544 8 ?g (r=0.999 9), 0.024 06～1.203 2 ?g (r =1), 0.011 06～0.552 8 ?g (r =1), 0.015 94～0.796 8 ?g (r =1), 0.009 104～0.455 2 ?g (r =1), 0.010 37～0.518 4 ?g (r=1) respectively. And the recovery rates of them were 101.06%, 100.22%, 101.54%, 99.42%, 100.21%, 101.65% respectively. Conclusion The method appeared to be simple, reliable, accurate and can be used to determine the contents of six phendic acids in Mailuoning injection.
