Preparation of tetrandrine-doxorubicin complex liposomes and its release property in vitro
- VernacularTitle:粉防己碱阿霉素脂质体的制备与体外释放度的研究
- Author:
Feng PAN
;
Chunmei HU
;
Lijun PAN
;
Chi WANG
- Publication Type:Journal Article
- Keywords:
tetrandrine-doxorubicin complex liposomes;
(NH4)2SO4-pH gradient method;
release
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Selecting doxorubicin and tetrandrine as model drug to prepare complex liposomes, study the methods of preparation, and research its release property in vitro. Methods The formulation of tetrandrine-doxorubicin complex liposomes was optimized by three different kinds of methods. And the optimum formula was selected through the orthogonal test according to the entrapment efficiency. Results Tetrandrine-doxorubicin complex liposomes were prepared by (NH4)2SO4-gradient method combined with pH gradient method. One optimum recipe was founded that tetrandrine-doxorubicin complex liposomes/ egg phosphatidyl choline was 1∶20, egg phosphatidyl choline/cholesterol was 3∶1, pH value was 7.6, incubation temperature was 50 ℃, concentration of (NH4)2SO4 was 250 mmol/L. The doxorubicin completely released within 24 h, and the tetrandrine released within 16 h. Conclusion Tetrandrine-doxorubicin complex liposomes have high entrapment efficiency with fine-looking, which is better for the further studies
