Construction and identification of the recombinant pCD-Eg95 plasmid of Echinococcus granulosus
- VernacularTitle:细粒棘球绦虫重组pCD-Eg95质粒构建及鉴定
- Author:
Hui ZHOU
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Recombinant pCD-Eg95 plasmid;
Construction;
Identification
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct and identify the recombinant pCD-Eg95 plasmid of Echinococcus granulosus. Methods: Total RNA was extracted from hydatid cyst protoscoleces shattered by supersound. The Eg95 coding gene was amplified by RT-PCR, and then was cloned into the eukaryotic expression vector pCDNA3.1 to construct the recombinant pCD-Eg95 plasmid. The recombinant plasmid was electroporated into E. coliBL21(DE3) strain. Results: The 471 bp Eg95 coding gene was successfully amplified, and the recombinant pCD-Eg95 plasmid was successfully constructed, which was confirmed by restriction endonuclease digestion and PCR. Conclusion: The results could provide the basis for the further research of the nucleic acid vaccine of Echinococcus granulosus.
