Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate
- VernacularTitle:烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中survivin基因及蛋白表达的研究
- Author:
Junhao XIE
- Publication Type:Journal Article
- Keywords:
Cigarette smoke condensate(CSC);
Immortalized human bronchial epithelial cells(BEP-2D);
survivin mRNA and protein;
Immunohistochemical;
Retro-translation PCR(RT-PCR)
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To study the change of the survivin mRNA and protein of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cell and BEP-2D cells treated by cigarette smoke condensate(CSC)for 15 weeks(P-15),25 weeks(P-25)and 38 weeks(P-38)were respectively chosen to study the survivin gene and protein by RT-PCR(retro-translation PCR,RT-PCR)and immunohistochemical method.Results:The survivin mRNA was found in BEP-2D cells of P-15,P-25 and P-38,respectively with 0.56,0.80,and 0.81,but not in the normal BEP-2D cell.The survivin protein was found in the normal BEP-2D cell,but not in BEP-2D cells of P-15,P-25 and P-38.The levels of survivin protein expressian were different between BEP-2D cells of P-15,P-25 and P-38 and normal BEP-2D cell,with significant difference between P-15 BEP-2D cell and normal BEP-2D cell(P
