Expressions of HMGB1mRNA and NF-?B in lung injury induced by intestinal ischemia-reperfusion in rats and protective effects of polydatin
- VernacularTitle:NF-?B和HMGB1在大鼠肠缺血再灌注肺损伤中的表达及白藜芦醇苷的保护作用
- Author:
Tingfang YANG
- Publication Type:Journal Article
- Keywords:
Intestinal ischemia-reperfusion(IIR);
Lung injury;
Nuclear factor-kappaB(NF-?B);
High mobility group box1(HMGB1);
Polydatin(PD)
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the activity of nuclear factor kappaB(NF-?B)and the gene expression of high mobility group box 1(HMGB1)in lung injury induced by intestinal ischemia-reperfusion(IIR),and to investigate the protective effect of polydatin(PD)in this lung injury.Methods:Fifty-four male Sprague-Dawley rats weighing 200~250 g were randomly divided into control group,IIR group(45 min intestinal ischemia with 0.5 h or 2 h or 6 h reperfusion)and PD group [IIR with PD(10 mg/kg)treatment].The level of pulmonary myeloperoxidase(MPO)was detected by colorimetric method,the expressions of NF-?B and ICAM-1 by immuneochemistry and that of pulmonary HMGB1mRNA by RT-PCR respectively.Results:Lung injury induced by IIR was characterized by pathological damage of edema,hemorrhage and inflammatory cell infiltration.Compared with control group,pulmonary W/D and MPO increased significantly(P
