The influencing factors for efficiency of liposome-mediated transfection of pReceiver-M29-PRKCB1 into human umbilical vein endothelial cell
- VernacularTitle:影响人脐静脉内皮细胞脂质体转染pReceiver-M29-PRKCB1效率因素的探讨
- Author:
Lian DUAN
- Publication Type:Journal Article
- Keywords:
Liposome;
Transfection;
Human umbilical vein endothelial cell
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective: To observe the influencing factors for efficiency of liposome-mediated transfection into human umbilical vein endothelial cell(HUVEC). Methods: Transferring HUVECs by distinct conditions,such as ratios of liposome and plasmid,densities of per well and different times of incubation,and transfection rates were observed and calculated by fluorescent microscope and flow cytometry. Results:⑴ When density of per well exceeded 2?l04 and times exceeded 4 hours,liposome-mediated transfection efficiency of endothelial cells decreased. ⑵With adding plasmid quality, transfection rates increased;while plasmid quality exceeded 1.0 ?g,in condition of 2?l04 per well and liposome volume 2 ?l, transfection efficiency reached the peak level. ⑶With adding liposome volume, transfection rates increased;while liposome volume was 8 ?l,in condition of 2?104 per well and plasmid quality 1 ?g, transfection efficiency was depressed.When ratios of liposome and plasmid were 1:6～1:8,the optimal transfection efficiency was 18.62%. Conclusion: Using optimal transfection parameter,we obtained the optimal transfection efficiency .
