Effect of administration by amniocentesis on chorioamnionitis and rophoblasts apoptosis in late pregnant rats with intrauterine infection by E coli
- VernacularTitle:宫内给药对晚孕大鼠细菌性宫内感染胎盘炎症与滋养细胞凋亡的影响
- Author:
Hao CHENG
- Publication Type:Journal Article
- Keywords:
Chorioamnionitis;
Escherichia coli;
Cell apoptosis;
Pregnancy trimester;
Administration by amniocentesis
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of ceftiaxone sodium administered by amniocentesis on chorioamnionitis and trophoblast apoptopsis in treatment of intrauterine infection with Escherichia coli(E coli)in late pregnant SD rats.Methods:36 SD pregnant rats for 15 days were randomly divided into four groups(9 in each),intrauterine admistration(IUA)group and control(IUC)group,maternal intravenous injection(IVA)group and control(IVC)group.0.2 ml E coli suspension at a dose of 1010/L was inoculated into one horn of uterus of each rat on 15-day of pregnancy.On the 16-day of pregnancy,each gestational sac in inoculation side of rats in IUA group and IUC group was given ceftriaxone sodium or appropriate volume of isotonic Na chloride(NS)by amniocentesis,and each rat in IVA group and IVC group was given ceftiaxone sodium or appropriate volume of isotonic NS intravenously.From the 17th day,3 rats in each group were sacrificed randomly and respectively everyday.The top 3 sacs in inoculation side of each rat were detected,placenta and membrane was get for histological analysis with light microscope by HE staining to compare Naeye's stage and grade of chorioamnionitis.One of the top 3 sacs in inoculation side of each rat was randomly selected.The apoptosis of trophoblasts was semiquantitatively analyzed by TUNEL staining.Results:The mild chorioamnionitis rates were higher in total and each day administration group of amniocentesis route,in total and late 2 days administration group of intravenous route,and in total and 1st day amniocentesis route group of two administration groups(P
