Inhibitory Effect of (-)Clausenamide on Apoptosis of PC12 Cells Induced by Serum Deprivation and Its Related Mechanism
- VernacularTitle:左旋黄皮酰胺抑制去血清所致神经细胞凋亡及其相关机制的研究
- Author:
Jinfeng HU
;
Na NING
;
Wei XUE
;
Juntian ZHANG
;
Naihong CHEN
- Publication Type:Journal Article
- Keywords:
(-)Clausenamide;
Serum deprivation;
PC12 cell;
Apoptosis
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of(-)clausenamide on the damage of PC12 cells induced by serum deprivation and to explore its related mechanism. Methods The cell viability was detected by MTT assay and morphological observation. The effect of(-)clausenamide on the PC12 cell apoptosis was analyzed by flow cytometry. Then western blotting and confocal microscope was used for the further study of effect of(-)clausenamide on the protein expression of GSK-3?,Bax and Bcl-2. Results(-)Clausenamide remarkably increased PC12 cell survival rate through inhibiting the PC12 cell apoptosis induced by serum deprivation at the concentration of 1 or 10 ? mol/L(P
