EXPERIMENTAL STUDY OF ACTIN AND MYOSIN IN THE SCAR TISSUE
- VernacularTitle:瘢痕中肌动蛋白及肌球蛋白的实验研究
- Author:
Qun ZHANG
;
Yunliang QIAN
;
Lei CUI
- Publication Type:Journal Article
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2001;15(1):32-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different expression of actin, myosin Ⅱ in hypertrophic scars, keloids and normal skins, and to understand the relationship of actin, myosin Ⅱ and the scar contracture. Methods Fifteen cases with hypertrophic scars, 10 cases with keloids and 15 cases with normal skins were chosen randomly. The expression of actin and myosin Ⅱ were detected by immunohistochemical method in the hypertrophic scars, keloids and normal skins. The fibroblasts isolated from three types of tissue were cultured in vitro, then actin and myosin Ⅱ in three different fibroblasts were measured using flow cytometry. Results The immunohistochemical staining of myosin Ⅱ in hypertrophic scars was positive, while the staining in keloids and normal skins were negative. The positive rate of myosin Ⅱ expression in hypertrophic scars, keloids and normal skins were (95.11±2.78)%， (16.86±7.11)%, and (5.31±1.79)% respectively. There were significant difference between keloids and the two others(P＜0.01). The actin expression in three difference tissues were positive, there were no significant difference in hypertrophic scars, keloids and normal skins(P>0.05). The positive rate of actin expression in hypertrophic scars, keoids and normal skins were(77.77±15.43)%, (88.89±10.29)%, and (82.92±13.48)% respectively, and there were no significant difference(P>0.05). Conclusion Myosin Ⅱ may play an important role in the scar contracture. Actin is the contractile protein of cell, it plays important role in cellular movement. Actin is necessary protein in the cell.
