Determination of Psoralen and Apigenin in Radix Fici Hirtae by HPLC
- VernacularTitle:HPLC法测定五指毛桃中补骨脂素和芹菜素的含量
- Author:
Ming LI
;
Zhiwei CHEN
;
Yucui LI
;
Zhenquan CHEN
;
Chuzhen CHEN
;
Ziren SU
- Publication Type:Journal Article
- Keywords:
Radix Fici Hirtae;
Psoralen;
Apigenin;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quantitative methods of psoralen and apigenin in Radix Fici Hirtae. Methods The content of psoralen and apigenin in Radix fici Hirtae was determined by HPLC. The chromatographic conditions were as follows:YMC C18 column(250 mm? 4.60 mm,5 ? m),mobile phases of methanol-0.2 % phosphoric acid for gradient elution,flow rate being 1.0 mL? min-1,column temperature at 30 ℃,detection wavelength being 245 nm for psoralen and 338 nm for apigenin,and inject volume being 10 ? L. Results Psoralen showed a good linear relationship in the range of 0.083 6～ 1.045 0 ? g,r=0.999 9,the average recovery was 100.43 % and RSD % was 0.45 % . Apigenin showed a good linear relationship in the range of 0.065 6～ 0.820 0 ? g,r=0.999 9,the average recovery was 100.41 % and RSD % was 0.34 % . Conclusion The established methods are simple and rapid with good reproducibility,and can be used for the quality control of Radix fici hertae.
