Determination of Acteoside in Conghuang Bushen Capsule by RP-HPLC
- VernacularTitle:HPLC法测定苁黄补肾胶囊中麦角甾苷的含量
- Author:
Yue SHI
;
Kaitong LI
;
Shuai YANG
- Publication Type:Journal Article
- Keywords:
Conghuang Bushen Capsule;
Acteoside;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method to determine the content of acteoside in Conghuang Bushen Capsule. Methods The HPLC method was employed with a column of Hypersil ODS2(4.6 mm? 250 mm,5 ? m) and a mobile phase of acetonitrile-methanol-1 % HOAc(13 ∶ 9 ∶ 78),the detection wavelength being at 334 nm,flow rate being 1.0 mL/min and the column temperature being at 40 ℃ . Results The linear range was 0.108 5 ? g ～ 0.650 7 ? g,r=0.999; 1.The average recovery of acteoside and RSD were 98.42 % and 1.89 %,respectively. Conclusion The present method is simple,rapid and reproducible. It can be used for quality control of Conghuang Bushen Capsule.
