Identification of multi-component in Danshen Dropping Pills by using a combinative LC/MS approach
- VernacularTitle:结合两种LC/MS方法用于复方丹参滴丸中多成分的鉴定
- Author:
Hongyang ZHANG
- Publication Type:Journal Article
- Keywords:
Danshen Dropping Pills;
time-of-flight mass spectrometer;
ion trap tandem mass spectrometer;
multi-component identification
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
AIM: To develop a combinative LC/MS for the identification of multi-component in Danshen Dro-(pping) Pills(Radix et Rhizoma Salviae miltiorrhizale,Radix et Rhizoma Notoginseng,Borneolum Syntheticum,etc.). METHODS: Separation was performed on a Phenomenex Luna C_(18) reverse-phase column(250 mm?4.6 mm,5 ?m).The mobile phase consisted of water containing 0.2% formic acid and acetonitrile was used as gradient elute.The flow rate was 1 mL/min.A combination of time-of-flight mass spectrometer(TOF-MS) and ion trap tandem mass spectrometer(Trap-MS~n) was applied for qualitative analysis under negative ion mode. RESULTS: Under optimized LC/MS condition,the molecular formulae of 14 components were screened by TOF-MS and 13 of them were then identified by Trap-MS~n.All of the components were surveyed and classified according to their medicinal materials derivation. CONCLUSION: The established combinative LC/MS method can thus be considered as an effective and credible pattern for the analysis of Chinese multi-herb remedy,as well as other complex systems.
