Effect of Tanshinone ⅡA on Content of NO and Activities of NOS and iNOS in Cerebral Ischemic Reperfusion Injury Rats
- VernacularTitle:丹参酮ⅡA对脑缺血再灌注损伤大鼠脑组织及血清中NO含量及NOS、iNOS活性的影响
- Author:
Hao LI
;
Kaixiang LIU
;
Junlin FENG
- Publication Type:Journal Article
- Keywords:
ischemiareperfusion injury;
NO;
NOS;
iNOS;
Tanshinone ⅡA;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effect of Tanshinone ⅡA (Tan ⅡA) on the content of NO and the activities of NOS and iNOS in cerebral ischemia reperfusion (I/R) injury rats,and explore its protective mechanism. Methods Rats were randomly divided into 4 groups,which were sham operated group,I/R group,low dose Tan ⅡA treated group and high dose Tan ⅡA treated group. The focal middle cerebral arterymocclusion (MCAO) model was made by suture-occluded method. Rats were pretreated with Tan ⅡA,ig for 3 d before MCAO. After 90 min MCAO following 24 h of reperfusion,pathomorphologic changes was investigated with HE staining. The content of NO and the activities of NOS and iNOS was also determined. Result The change of ischemic impairment in low or high dose Tan ⅡA treated group was lighter than that of I/R group,and high dose Tan ⅡA treated group was lighter than that of low dose Tan ⅡA treated group. Compared with sham operated group,the content of NO and the activities of NOS and iNOS increased at 24 h of reperfusion in the ischemic territory (P
