Content Determination of Active Component in Epimedium Koreanum Nakai of Different Pharmaceutical Genetic in Liaoning Province
- VernacularTitle:辽宁产朝鲜淫羊藿不同药用部位有效成分含量的测定
- Author:
Na SHAN
;
Jincai LU
;
Jing WANG
- Publication Type:Journal Article
- Keywords:
Epimedium koreanum Nakai;
total flavonids;
icariin;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of icariin and flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic in Liaoning Province. Methods Icariin was used as the chemical reference substance to establish HPLC and ultraviolet spectrophotometry methods for content determination of icariin and total flavonoids. Results The content of icariin in leaves was from 0.197% to 0.347%,and in stem was from 0.043% to 0.050%. The content of total flavonids in leaves was from 6.68% to 8.85%,and in stem was from 1.3% to 2.7%. The calibration curves of icariin was in good linearity over the range of 0.3～1.8 ?g (r=0.999 9) and total flavonids was 0.5～3 ?g/mL (r=0.999 9). The average recovery of icariin was 99.43% (n=6,RSD=0.29%) and total flavonids was 100.38% (n=9,RSD= 1.60%). Conclusions The content of flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic has large difference,the content in leaves is far higher than in stem.
