Determination of Syringin in Huoxue Tongluo Pill by HPLC
- VernacularTitle:高效液相色谱法测定活血通络丸中紫丁香苷的含量
- Author:
Gang WANG
;
Caide ZHAO
;
Yuxun MA
- Publication Type:Journal Article
- Keywords:
Huoxue Tongluo Pill;
HPLC;
syringin;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the content determination of syringin in Huoxue Tongluo Pill. Methods Chromatographic assay was performed on Kromasil C18 column (250 mm?4.6 mm,5 ?m) at room temperature,and the mixture solution of acetonitrile-1% aqueous acetic acid (10∶90,v/v) was used as mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was at 270 nm. The sample size was 20 ?L. Results Good linear relationship was achieved when the concentration of syringin was in the range of 5.3～53 ?g/mL. The average recovery was 98.29% with RSD=1.71%. Conclusion The method is simple,accurate and the results are stable with good reproducibility. This method can be used for the content determination of syringin in Huoxue Tongluo Pill.
