Determination of Peoniflorin and Glycyrrhizic Acid in Modified Shaoyao Gancao Granules by High Performance Liquid Chromatography
- VernacularTitle:高效液相色谱法检测加味芍药甘草颗粒中芍药苷、甘草酸的含量
- Author:
Yan LU
;
Tuohua PENG
- Publication Type:Journal Article
- Keywords:
MODIFIED SHAOYAO GANCAO GRANULES/chemistry;
PEONIFLORIN/analysis;
GLYCYRRHIZIC ACID/anlysis;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of peoniflorin and glycyrrhizic acid in modified Shaoyao Gancao Granules.Methods Reversed-phase high performance liquid chromatography(HPLC) was used.The chromatographic condition was as follows: Diamonsil C18 column(4.6mm ?250mm,5?m),column temperature being room temperature,mobile phase consisting of acetonitrile(A)-10g/L acetic acid(B) with adjusting pH value being 3.5,flowing rate at 1.0mL/min,detective wavelength at 250nm,and the injection volume being 20?L.The gradient elution procedure was as follows: elution with 18% A for 0～5 min,elution with 19%～29% A for 6～10 min,elution with 30%～45% A for 11～25min,and A+B=100%.Results Peoniflorin at the concentration of 1.916～122.6?g/L and glycyrrhizic acid at the concentration of 2.625～168?g/L showed a good linearity.The mean recovery was 101.12% for peoniflorin and 99.68% for glycyrrhizic acid.Conclusion This method is simple and reproducible,and can be used for the determination of peoniflorin and glycyrrhizic acid in modified Shaoyao Gancao Granules.
