HPLC Fingerprint of Radix Gentianae Crassicaulis
- VernacularTitle:粗茎秦艽药材HPLC指纹图谱研究
- Author:
Yanyan WANG
;
Zhili ZHAO
;
Jinrong WU
;
Zhengtao WANG
- Publication Type:Journal Article
- Keywords:
Radix Gentianae Crassicaulis;
fingerprint;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC fingerprint of Radix Gentianae Crassicaulis.Methods The fingerprints were obtained on an Ultimate XB-C18 column(250 mm?4.6 mm,5 ?m)with the gradient elution solvent system composed of acetonitrile and 0.05% phosphoric acid.The flow rate was 1.0 mL/min,the column temperature was maintained at 25 ℃,and the detection wavelength was set at 240 nm.Results Ten common peaks were selected as the fingerprint peaks and the fingerprints also were evaluated by the Similarity Evaluation System for Chromatographic Fingerprint with the correlation coefficient of 0.98—1.0.Conclusion This method with good precision and reproducibility is reliable for the quality control of Radix Gentianae Crassicaulis.
