Effects of celiac ganglion damage on expression of substance P in spinal cord of gastric distension rabbits
- VernacularTitle:腹腔神经节毁损对胃扩张兔脊髓P物质表达的影响
- Author:
Lingquan ZENG
- Publication Type:Journal Article
- Keywords:
Celiac ganglion;
Substance P;
Spinal cord;
Gastric distension;
Pain
- From:
Journal of Chongqing Medical University
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the influence of damaging celiac ganglion(CG)by high intensity focused ultrasound(HIFU)on expression of substance P(SP) in spinal cord following gastric distension in rabbits,and study whether SP participate in transferring pain information as neurotransmitter. Mothods:20 rabbits were randomly divided into four groups in a 2 ?2 factorial design:not distended not damaged(nDnB),distended not damaged(DnB),not distendedly damaged(nDB),distendedly damaged(DB),with 5 in each group. At first,group nDB and DB were damaged by HIFU,group nDnB and DnB not damaged. 7 days later,they all were installed with the device of gastric distension. In group nDB and nDnB,tissue were directly fixed through infusing 4% parafomaldehyde under deep anaesthesia,then spinal cord of thoracic 6￣8(T6￣T8) was extracted. In group DB and DnB stomach was distended for 1 h,and the spinal cord were extracted in the way similar to that in the above two groups. Immunohistochemistry technique of strept avidin biotin complex(SABC)was used to display the content of SP in spinal cord. Results:In lamina and Ⅱ of dorsal horn,damaged CG could induce the OD in afferent projections and medial zone to increas significantl(yP
