Preliminary study on cancer stem-like cells(side population)in human colorectal cancer cell lines
- VernacularTitle:结直肠癌细胞系肿瘤干细胞相关亚群初步研究
- Author:
Xiang HU
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Cell line;
Cancer stem cell
- From:
Journal of Chongqing Medical University
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the content of cancer stem-like cells in different human colorectal cancer ce11 lines and explore the methods for enriching cancer stem-like cells in SW480.Methods:The percentage of side population cells(SP)in human colorectal cancer cell lines LOVO,SW480,HCT116 were detected by flow cytometry.SW480 cell line was cultivated in serum-free medium(SFM)supplemented with growth factors and cancer stem-like cells reforming into floatingspheres were isolated.The isolated cancer stem-like cells were identified by limiting-dilution assay,differentiation assay,self-renewal assay,alternative cultivation assay.Results:The percentage of SP cells was 1.0% in LOVO,1.2% in SW480 and 1.3% in HCT116.In the absence of serum,a minority(0.54%￣0.62%)of cancer stem-like cells in SW480 cells survived,proliferated and assembled into the suspended tumor cell spheres.SW480 cancer stem-like cells possessed proliferative,self-renewal and differentiation potential,which were responsible for the floating tumor clone.Serum addition into SFM resulted in the proliferation of cancer stem-like cells;after several generations and alternated cultivation in SSM and SFM,cancer stem-like cells maintained their characters.Conclusion:LOVO,SW480 and HCT116 cell lines contain a tiny minority of SP cells with stem cell properties.The cancer stem-like cells in SW480 line can be maintained in SFM using a floating culture method.
