Curcumin inhibits proliferation of lung cancer cell A549 through Wnt signaling pathway
- VernacularTitle:姜黄素通过Wnt信号转导通路抑制肺癌细胞A549增殖的研究
- Author:
Xiu YUE
- Publication Type:Journal Article
- Keywords:
Curcumin;
?-catenin;
Wnt signaling pathway;
A549 cell
- From:
Journal of Chongqing Medical University
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the possible molecular mechanism of inhibiting the proliferation of lung cancer cells by studing curcumin regulating neucleoprotein ?-catenin,the key factor of Wnt signaling pathway.Methods:Non-toxic different doses of curcumin affected human lung adenocarcinoma cell A549 for 48 hours.Inhibitory effect of curcumin on proliferation of A549 cells was evaluated by MTT assay.The correclation of doses and inhibition ratio was analyzed statistically.The neucleoprotein levels of ?-catenin was analyzed by Western blot.The expression level of c-myc was analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The exchange of cell cycle was examined by flow cytometry(FCM).Results:Curcumin inhibited the proliferation of A549 in a concentration-dependent manne(rP
