Quality standard for Compound Shenyi Granule
- VernacularTitle:神怡颗粒的质量标准研究
- Author:
Rui AN
;
Sili ZHOU
;
Lisha YOU
;
Xinhong WANG
- Publication Type:Journal Article
- Keywords:
Compound Shenyi Granule;
TLC;
glycyrrhizic acid;
glycyrrhetinic acid;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish quality standard for Compound Shenyi Granule(Fructus Jujubae,Radix et Rhizoma Glycyrrhizae,Radix et Rhizoma Salviae miltiorrhizae,Radix Rehmanniae).METHODS:Ziziphus jujuba Mill、Glycyrrhiza uralensis Fisch、Salvia miltiorrhiza Bge、Rehmannia glutinosa Libosch.were identified by TLC.Glycy-rrhizic acid and glycyrrhetinic acid were determined by HPLC.The chromatographic separation was performed on an Agilent ZORBAX Eclipse?DB-C_ 18(4.6 mm?250 mm,5 ?m)column by a gradient elution with methanol and 2.5 % acetic acid at a flow-rate of 1.0 mL/min;the detection wavelength was set at 254 nm.RESULTS:TLC spots developed were fairly clear and the blank test showed no interference.The linear rangers were 1.135-9.08 ?g(r=0.999 3) for glycyrrhizic acid,0.71-5.68 ?g(r=0.999 7) for glycyrrhetinic acid,respectively.CONCLUSION:The methods is effective for the quality control of Compound Shenyi Granule.
