Effects of Serum Contained Erzhi Tiangui Granule on Secretion Function of Ovarian Granulosa Cells from Primary Senile Mice
- VernacularTitle:二至天癸颗粒含药血清对初老小鼠卵巢颗粒细胞分泌功能的影响
- Author:
Lin WANG
;
Fang LIAN
;
Fengmei MA
- Publication Type:Journal Article
- Keywords:
Erzhi Tiangui Granule;
ovarian granulosa cell;
estradiol;
progesterone;
inhibin B;
serum contained drug
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of serum contained Erzhi Tiangui Granule on the secretion function of ovarian granulosa cells from primary senile mice. Methods The serum with decoction from primary senile mice was added to cultured granulosa cells and incubated for 48 h. Then the concentration of estradiol (E2), progesterone (P) and inhibin B (INHB) in cultured medium were measured by chemiluminescence immunoassay (CLIA) and Sandwich ELISA respectively. Results The serum contained Erzhi Tiangui Granule could increase the secretion of E2, P and INHB. Conclusion Erzhi Tiangui Granule can improve the ovarian function of primary senile mice by regulating the secretion function of granulosa cells.
