EXPRESSIONS OF TRANSFORMING GROWTH FACTOR-BETA 1 AND ITS TYPE Ⅱ RECEPTOR IN EXPERIMENTAL RAT ASCENDING AORTIC ANEURYSM
- VernacularTitle:转化生长因子?1及其2型受体在实验性大鼠升主动脉瘤中的表达
- Author:
Fang LIU
;
Shuling BAI
;
Jun FAN
;
Jun WANG
;
Hao TONG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-beta 1;
Transforming growth factor-beta receptor 2;
Aortic aneurysm;
Immunohistochemistry;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions and significance of transforming growth factor-beta 1(TGF?1) and its typeⅡ receptor(TGF?RⅡ) in experimental rat ascending aortic aneurysm of rat model.Methods The rat ascending aortic aneurysm models were made by banding ascending aorta of young Wistar rats.The ascending aortas were taken 4 months after operation.Immunohistochemistry staining and Western blotting were used to investigate the expressions of TGF?1 and TGF?RⅡ.Result Immunohistochemistry staining results showed that TGF?1 expressed in all layers of the aortic aneurysm and the control.TGF?RⅡ was extensively located in the hyperplastic intima and tunica media smooth muscle cells in the aortic aneurysm,while there was only a little positive staining in the control group.Western blotting results indicated that the expression levels of TGF?1 and TGF?RⅡ in the aortic aneurysm were stronger than the control,P
