EFFECTS OF IGFBP-3/RXR? OR STAT-1 SIGNALING PATHWAY ON A?_(1-42) INDUCED APOPTOSIS IN RAT HIPPOCAMPUS NEURONS
- VernacularTitle:与IGFBP-3相关的RXR?、STAT-1信号通路在?-淀粉样肽_(1～42)诱导大鼠海马神经元凋亡中的可能作用
- Author:
Limin CHEN
;
Xiaochun CHEN
;
Xiaodong PAN
- Publication Type:Journal Article
- Keywords:
?-amyloid_(1-42);
Apoptosis;
Insulin-like growth factor-binding protein-3(IGFBP-3);
Retinoid X receptor-?(RXR?);
STAT-1;
Hippocampus neuron;
Immunofluorescence;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of IGFBP-3,RXR? and STAT-1 on A?_ 1-42 induced apoptosis in rat hippocampus neurons.Methods Apoptosis was induced by fibrillar A?_ 1-42.The percentage of neurons apoptosis was evaluated by microscopy after staining with TUNEL/DAPI.IGFBP-3 and RXR? positive neurons were observed by immunofluorescence.The expression of RXR? and STAT-1 protein were detected by Western blotting.Results After treatment with 20?mol/L A?_ 1-42 for 24 hours,the apoptotic hippocampus neurons were shown by TUNEL/DAPI assay.The percentage of apoptotic neurons was increased in a time-dependent manner.During the development of apoptosis,both the percentage of IGFBP-3/RXR? positive neurons and the expression of RXR? protein increased markedly after 3-6hours(P
