Expression and activity detection of IL3-PE38KDEL
- VernacularTitle:免疫毒素IL-3-PE38KDEL的表达及活性测定
- Author:
Yan SHEN
- Publication Type:Journal Article
- Keywords:
Human interleukin-3;
Pseudomonas exotoxin(PE38KDEL);
Immunotoxin
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a new type of anti-leukemia immunotoxin with killing activity.Methods:The method of cytotoxicity was used to study the activity of the immunotoxin after the induction of IPTG. Results:The expressed fusion proteins were detected mostly as inclusion bodies at high level.The result showed IL3-PE38KDEL had liable activity of toxicity. Conclusion:The fusion protein IL3-PE38KDEL has good biological activity,which paves way for the further study on its treatment of leukemia.
