Effects of losartan on PKC-ERK_1/ERK_2 and cardiac remodeling after my-ocardial infarction in rats
- VernacularTitle:氯沙坦干预对急性心梗大鼠PKC-ERK_1/ERK_2及心室重塑的影响
- Author:
Jin ZHANG
- Publication Type:Journal Article
- Keywords:
Myocardial infarction;
Remodeling;
Angiotensin II;
Protein kinase C;
MAPK
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of losartan on the changes of expression of signal molecule PKC、ERK1/ERK2,and p-ERK1/ERK2 in non-infarction zone of left ventricule(LVNIZ)and left ventricular remodeling(LVRM)in rat with acute my- ocardial infarction(AMI). Methods:The 36 surviving AMI male Wistar rats were randomly divided into 2 groups:AMI group(n= 30)(subdivided into 5 groups according to the different intervals at 1,3,7,14,28 days after AMI.) and losartan group(n=6) (by gastric gavate,20mg(/kg?d),for 4 weeks). used Sham 7d group(n=6) was used as controls. LVEDP was analyzed by ALC -MPA biological signal analysis system. LVWI was measured when the hearts were harvested. The concentration of plas- ma and non-infarcted myocardial tissue AngⅡ were measured with radioimmunoassay. The expression of PKC, ERK1/ERK2, and p -ERK1/ERK2 protein in remote non -infarcted myocardium were evaluated by Western blot. Results:Compared with Sham-operated rats,LVEDP、LVWI and the concentration of LVNIZ AngⅡ in the 28th day after MI group were all markedly increased(P
