Effect of ketamine on the expression of hepatic stress protein IFIT1 and JAB1 during the early stage of burns in mice
- VernacularTitle:氯胺酮对烧伤早期小鼠肝应激相关蛋白IFIT1和JAB1表达的影响
- Author:
Jiping GONG
- Publication Type:Journal Article
- Keywords:
Interferon-induced protein with tetratricopetide repeats1(IFIT1);
c-Junactivationdomain-bindingprotein1(JAB1);
Systemic inflammatory response syndrome(SIRS)
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To evaluate the effect of ketamine on the expression level of hepatic stress protein IFIT1 and JAB1 during the early stage of burns in mice, and observe the location of IFIT1 and JAB1 in hepatic cells. Methods:15 C57/129 male mice were divided randomly into three groups(n=5): normal control,burns,burns+ketamine. Burns group and burns+ketamine group were inflicted with 15%~20% TBSA full thickness burn injury,and burns+ketamine group received an intramuscular injection of 10 mg/kg ketamine 15 min after burns. At 4 h after burns,hepatic tissue was taken from mice,and the levels of hepatic I- FIT1 and JAB1 were detected by western blot. Normal control hepatic pathological section was taken; then cell location of I- FIT1 and JAB1 was detected by immunohistochemistry. Results:In burns group, the expression level of hepatic IFIT1 signifi- cantly increased,while that of JAB1 decreased as compared with normal control(P
