Determination of Verbascoside in Mitonghua Granules by HPLC
- VernacularTitle:HPLC法测定蜜桶花颗粒中毛蕊花糖苷的含量
- Author:
Ming YIN
;
Juan MOU
;
Huafen XIAO
;
Lirong DONG
;
Zongshun LI
- Publication Type:Journal Article
- Keywords:
Mitonghua Granules;
Verbascoside;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for determining verbascoside in Mitonghua Granules by HPLC. Method Verbascoside was determined by HPLC method,Agilent ZORBAX-Extend C18 column (4.6 mm?150 mm,5?m) with Acetonitrile-1 %Acetic acid(13∶87) as the mobile phase was used for elution.The flow rate was at 1.0 mL/min and the detective wavelength was at 334 nm. The column temperature was at 30 ℃. Results There was a good linearity relationship at the range of 0.062 8～1.256 0 ?g for verbascoside (r=0.999 83) .The average recovery was 99.61 %,and RSD was 1.55 %(n=6). Conclusion The method is simple,reproducible and accurate with a good repeatability,and can be used for the quality control of Mitonghua Capsules.
