Effects of Tianma Gouteng Yin on Proliferation of Cardiac Fibroblasts of Spontaneous Hypertensive Rat Stimulated by Insulin
- VernacularTitle:天麻钩藤饮对胰岛素促自发性高血压乳鼠心肌成纤维细胞增殖的影响
- Author:
Xiwen HUANG
;
Jinghe SUN
;
Yuping HE
;
Shaoxiang XIAN
- Publication Type:Journal Article
- Keywords:
Tianma Gouteng Yin;
Cardiac fibroblasts;
Insulin;
Spontaneous Hypertensive Rats;
Proliferation
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Tianma Gouteng Yin (TGY) on the proliferation of myocardial fibroblasts of spontaneous hypertensive rat(SHR) stimulated by insulin. Methods The cardiac fibroblasts from one-day-old spontaneously hypertensive rat were primarily cultured and then sub-cultured to 3rd-6th generation,and were identified with light microscopy and immunohistochemical technique. The effect of serum containing TGY on the proliferation of cardiac fibroblasts of SHR treated with insulin was determined by MTT colorimetric assay and 3-H-TdR incorporation,and the cell cycle was analysised with flow cytometer. Results TGY inhibited the proliferation of cardiac fibroblasts induced by insulin. Insulin can stimulate the proliferation of fibroblasts by promoting the cell cycle transfer from G0/G1 period to S period and increasing the synthesis of cell DNA. Compared with the model group,the percentage of the cells at G0/G1 period was much higher,while the percentage of the cells at S period was much lower in TGY group. Conclusion TGY has the effect on inhibiting the proliferation of cardiac fibroblasts and inhibiting the cell cycle transfer from G0/G1 period to S period,which may be one of its therapeutic and protective mechanisms for hypertension and myocardial fibrosis in clinic.
