HPLC Fingerprint Analysis for Flos Daturae
- VernacularTitle:洋金花药材HPLC指纹图谱分析
- Author:
Li ZHANG
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
Flos Daturae;
fingerprint;
scopolamine;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC fingerprint analysis method for the quality control of Flos Daturae. Methods Column:Zorbax C18 (4.6 mm?250 mm, 5 ?m);Mobile phase:0.07 mol/L NaPO4 (adjust pH to 6.0 with phosphatic acid, including 17.5 mmol Soldium dodecyi Sulphate)-Acetotrile (2∶1). Flow rate was 0.8 mL/min and detection wave length was 216 nm. Results The repetition and precision of the method was good. There was obvious difference between South and North Flos Daturae based on the results of cluster and similarity analysis. Conclusion The method can control the quality of Flos Daturae effectively.
