Effects of ?-asarone on Mitochondrial Membrane Potential of Cardiac Myocytes with Ischemia Reperfusion Injury
- VernacularTitle:?-细辛醚对缺血再灌注损伤心肌细胞线粒体膜电位的影响
- Author:
Qiwen WANG
;
Qiduan WU
;
Yizhi CHEN
- Publication Type:Journal Article
- Keywords:
?-asarone;
Cardiac myocytes;
Ischemia reperfusion injury;
Mitochondrial membrane potential
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of ?-asarone on mitochondrial membrane potential(MMP) of cardiac myocytes with ischemia reperfusion injury. Methods The cardiac myocytes model of myocardial ischemia reperfusion injury (MIRI) was induced with sodium dithionite (Na2S2O4). The primary cultured cardiac myocytes of SD neonatal rats were randomized into six groups:normal group,model control group,high-dose ?-asarone (25 ?g/mL),middle-dose ?-asarone group(12.5 ?g/mL),low-dose ?-asarone group (6.25 ?g/mL) and medium group. Mitochondrial membrane potential(MMP) of myocardial cells were measured by flow cytometry. Results Compared with the normal group,MMP in the model control group was obviously decreased(P
